OCLN Polyclonal Antibody for Human(Homo sapiens) | EIAab.comOCLN Polyclonal Antibody for Human(Homo sapiens)

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Index > Protein center > OCLN(Gene name) > Human

OCLN (Gene name),

Occludin (Protein name ), OCLN_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

OCLN;

Protein name:

Occludin;

Alternative:

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with TJP1/ZO1 and with VAPA.

Function:

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.

Subcellular Location:

Membrane Multi-pass membrane protein Cell junction tight junction

Protein Attributes:

Sequence length:

522

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

522:

KQLKSKLSHI | KKMVGDYDRQ | KT

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

251290

SMR:

Q16625

KEGG:

hsa:100506658

UniGene:

Hs.592605

String:

Q16625

STRING:

9606.ENSP00000347379

Pfam:

PF01284

Uniprot:

Q16625

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ELISA Kit for Human Occludin

Cat.:

E14928h

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Packing:

96T

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ELISA Kit for Human Occludin

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ELISA Kit for Human Occludin

Cat.:

E14928c

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96T

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ELISA Kit for Human Occludin

MSDS:

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ELISA Kit for Human Occludin

Cat.:

E14928m

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Packing:

96T

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CLIA Kit for Human Occludin

Cat.:

U14928m

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Packing:

96T

CLIA Kit for Human Occludin

MSDS:

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CLIA Kit for Human Occludin

MSDS:

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CLIA Kit for Human Occludin

MSDS:

Please sign in first.

CLIA Kit for Human Occludin

Cat.:

U14928h

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human Occludin

Monoclonal Antibody for Human Occludin

Protein for Human Occludin

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of OCLN into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-103
Plasma	97	93-106

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OCLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-102%	90-103%	93-98%	91-100%
EDTA plasma(n=5)	90-106%	93-101%	91-102%	93-102%
heparin plasma(n=5)	93-103%	94-100%	92-100%	91-104%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues."
Ando-Akatsuka Y. , Saitou M. , Hirase T. , Kishi M. , Sakakibara A. , Itoh M. , Yonemura S. , Furuse M. , Tsukita S.
J. Cell Biol.133:43-47(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
tissue: Colon carcinoma.
2.

"Occludin confers adhesiveness when expressed in fibroblasts."
Van Itallie C.M. , Anderson J.M.
J. Cell Sci.110:1113-1121(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);DOMAIN;TOPOLOGY
tissue: Liver.
3.

"Splicing diversity of the human OCLN gene and its biological significance for hepatitis C virus entry."
Kohaar I. , Ploss A. , Korol E. , Mu K. , Schoggins J.W. , O'Brien T.R. , Rice C.M. , Prokunina-Olsson L.
J. Virol.84:6987-6994(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 4; 5; 6 AND 7);ALTERNATIVE SPLICING
tissue: Liver.
4.

"Genomic structure of occludin gene."
Fukasawa M. , Toyota T. , Yoshitsugu K. , Yoshikawa T.
Submitted (2001-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N. , Kawamura Y. , Fukumoto A. , Miura A. , Honma R. , Satoh R. , Wakamatsu A. , Yamamoto J. , Kimura K. , Nishikawa T. , Andoh T. , Iida Y. , Ishikawa K. , Ito E. , Kagawa N. , Kaminaga C. , Kanehori K. , Kawakami B. , more...
Nat. Methods5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
6.

"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J. , Martin J. , Terry A. , Couronne O. , Grimwood J. , Lowry S. , Gordon L.A. , Scott D. , Xie G. , Huang W. , Hellsten U. , Tran-Gyamfi M. , She X. , Prabhakar S. , Aerts A. , Altherr M. , Bajorek E. , Black S. , more...
Nature431:268-274(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
7.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)
tissue: Brain.
tissue: Lung.
8.

"Occludin TM4(-): an isoform of the tight junction protein present in primates lacking the fourth transmembrane domain."
Ghassemifar M.R. , Sheth B. , Papenbrock T. , Leese H.J. , Houghton F.D. , Fleming T.P.
J. Cell Sci.115:3171-3180(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ALTERNATIVE SPLICING (ISOFORM 2)
9.

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S. , Ballif B.A. , Smogorzewska A. , McDonald E.R. III , Hurov K.E. , Luo J. , Bakalarski C.E. , Zhao Z. , Solimini N. , Lerenthal Y. , Shiloh Y. , Gygi S.P. , Elledge S.J.
Science316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Embryonic kidney.
10.

"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G. , Ye M. , Zhou H. , Jiang X. , Feng S. , Jiang X. , Tian R. , Wan D. , Zou H. , Gu J.
Proteomics8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Liver.
11.

"Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions."
Elias B.C. , Suzuki T. , Seth A. , Giorgianni F. , Kale G. , Shen L. , Turner J.R. , Naren A. , Desiderio D.M. , Rao R.
J. Biol. Chem.284:1559-1569(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH TJP1;PHOSPHORYLATION AT TYR-398 AND TYR-402;MUTAGENESIS OF TYR-398 AND TYR-402;SUBCELLULAR LOCATION
12.

"Density-enhanced phosphatase 1 regulates phosphorylation of tight junction proteins and enhances barrier function of epithelial cells."
Sallee J.L. , Burridge K.
J. Biol. Chem.284:14997-15006(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION;DEPHOSPHORYLATION BY PTPRJ;SUBCELLULAR LOCATION
13.

"PKC eta regulates occludin phosphorylation and epithelial tight junction integrity."
Suzuki T. , Elias B.C. , Seth A. , Shen L. , Turner J.R. , Giorgianni F. , Desiderio D. , Guntaka R. , Rao R.
Proc. Natl. Acad. Sci. U.S.A.106:61-66(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SUBCELLULAR LOCATION;PHOSPHORYLATION AT THR-403 AND THR-404;MUTAGENESIS OF THR-404
14.

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T. , Prokhorova T.A. , Akimov V. , Henningsen J. , Johansen P.T. , Kratchmarova I. , Kassem M. , Mann M. , Olsen J.V. , Blagoev B.
Sci. Signal.4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321; SER-369 AND SER-370;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
15.

"Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach."
Sundstrom J.M. , Tash B.R. , Murakami T. , Flanagan J.M. , Bewley M.C. , Stanley B.A. , Gonsar K.B. , Antonetti D.A.
J. Proteome Res.8:808-817(2009) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 416-522;PHOSPHORYLATION AT SER-490
16.

"Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyria."
O'Driscoll M.C. , Daly S.B. , Urquhart J.E. , Black G.C. , Pilz D.T. , Brockmann K. , McEntagart M. , Abdel-Salam G. , Zaki M. , Wolf N.I. , Ladda R.L. , Sell S. , D'Arrigo S. , Squier W. , Dobyns W.B. , Livingston J.H. , Crow Y.J.
Am. J. Hum. Genet.87:354-364(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANT BLCPMG SER-219

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